Extraction, separation and biological activities of some secondary metabolites from the plant Cytisus purgans

Abstract

In this study, Seseli praecox of Algerian flora was investigated regarding their polyphenolic and flavonoids contents using the Folin–Ciocalteu and aluminum chloride colorimetric methods, respectively. TPC of crude aqueous ethanolic extract was 44mg± 0.09GAE/gDw, while, TFC have values of 1.53mg±0.01 QuercetinE/gDw and 6.93mg±0.01 RutinE/gDw for ethanolic extract, which mean that this plant is relatively poor source of polyphenols and flavonoids . In the other side, the polyphenolic extract of this plant was evaluated for its reducing power. The capacity of the extract to reduce the ferricferricyanide complex to the ferrous-ferricyanide complex mention that Seseli praecox showed a very high iron reducing power activity with Ec50=0.25mg/ml.